App ID: FLM-C220017
Comixsil HCS利用陽(yáng)離子交換與氫鍵作用分離核苷（MS匹配)
1. Thymidine(胸苷)
2. Uridine(尿苷)
3. Deoxyadenosine(脫氧腺苷)
4. Adenosine(腺苷)
5. Deoxyguanosine(脫氧鳥(niǎo)苷)
6. Guanosine(鳥(niǎo)苷)
Column: Comixsil HCS 3u 100*3.2mm (P/N: FMD-HCS3-RONU)
Mobile phase of channel C preparation:
Place 50 ml of formic acid in 1 L volumetric flask. Add 800 ml of ACN and stir. Add 5 g of ammonium formate and stir until all ammonium formate is dissolved. Dilute to 1 L with ACN. This will create 5% formic acid and 0.5% ammonium formate in ACN to be used in channel C.
Q-Pump set up:
A: ACN: B: MeOH C: 5% formic acid with 0.5% ammonium formate in ACN
Gradient set up:
Time min A% B,% C%
0 88 10 2
8 48 50 2
8.01 88 10 2
Run time 16 minutes
Flow rate: 0.8 ml/min
Detection: 275nm
本應(yīng)用適用于核苷在質(zhì)譜檢測(cè)器下的分析
